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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Sodium Potassium ATPase Rabbit mAb

Sodium Potassium ATPase Rabbit mAb#48913

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Product Detail

Product NameSodium Potassium ATPase Rabbit mAb

Clone No.ST0533

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic peptide within Human ATP1A1 aa 39-83 / 1023.

ConjugateUnconjugated

Other NamesATPase Na+/K+ transporting alpha antibody
adenosinetriphosphatase antibody
AT1A1_HUMAN antibody
ATP1A1 antibody
ATP1A4 antibody
ATP1AL2 antibody
ATP1B antibody
ATP1B1 antibody
ATPase Na+/K+ transporting alpha 1 polypeptide antibody
ATPase Na+/K+ transporting alpha 4 polypeptide antibody
ATPase Na+/K+ transporting beta 1 polypeptide antibody
ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
Beta 1-subunit of Na(+),K(+)-ATPase antibody
Na(+)/K(+) ATPase alpha-1 subunit antibody
Na(+)/K(+) ATPase alpha-4 subunit antibody
Na+, K+ ATPase alpha subunit antibody
Na+/K+ ATPase 1 antibody
Na+/K+ ATPase 4 antibody
Na+/K+ ATPase, alpha-D polypeptide antibody
Na, K-ATPase beta-1 polypeptide antibody
Na, K-ATPase, alpha-A catalytic polypeptide antibody
Na,K-ATPase catalytic subunit alpha-A protein antibody
Na,K-ATPase subunit alpha-C antibody
polypeptide-like 2 antibody
Sodium pump 1 antibody
sodium pump 4 antibody
Sodium pump subunit alpha-1 antibody
sodium pump subunit alpha-4 antibody
sodium pump subunit beta-1 antibody
sodium-potassium ATPase catalytic subunit alpha-1 antibody
sodium-potassium ATPase catalytic subunit alpha-4 antibody
sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
sodium-potassium ATPase, alpha 4 polypeptide antibody
sodium-potassium-ATPase, alpha 1 polypeptide antibody
Sodium/potassium transporting ATPase alpha 1 chain antibody
Sodium/potassium transporting ATPase subunit beta 1 antibody
sodium/potassium-dependent ATPase beta-1 subunit antibody
sodium/potassium-transporting ATPase alpha-4 chain antibody
sodium/potassium-transporting ATPase beta-1 chain antibody
Sodium/potassium-transporting ATPase subunit alpha-1 antibody
sodium/potassium-transporting ATPase subunit alpha-4 antibody

Accession NoSwiss-Prot#:P05023

Uniprot P05023

Gene ID 476;

Calculated MW100 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin.
ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Sodium Potassium ATPase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Sodium Potassium ATPase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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