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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MSI2 Rabbit mAb

MSI2 Rabbit mAb#48916

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Product Detail

Product NameMSI2 Rabbit mAb

Clone No.ST59-02

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesFLJ36569 antibody
MGC3245 antibody
Msi2 antibody
MSI2/HOXA9 fusion gene, included antibody
MSI2H antibody
MSI2H_HUMAN antibody
Musashi 2 antibody
Musashi homolog 2 antibody
Musashi RNA binding protein 2 antibody
Musashi, Drosophila, homolog of, 2 antibody
Musashi-2 antibody
RNA binding protein Musashi homolog 2 antibody
RNA-binding protein Musashi homolog 2 antibody
WD 40 repeat protein MSI2 antibody

Accession NoSwiss-Prot#:Q96DH6

Uniprot Q96DH6

Gene ID 124540;

Calculated MW35/37 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of MSI2 on different lysates using anti-MSI2 antibody at 1/1,000 dilution. Positive control:
Lane 1: Lovo
Lane 2: PC-12
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
ICC staining MSI2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MSI2 in Lovo cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with MSI2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to post-translational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.

If you have published an article using product 48916, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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