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Location： Home > Recombinant Rabbit Monoclonal Antibodies > JNK2 Rabbit mAb

JNK2 Rabbit mAb#48922

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Product Detail

Product NameJNK2 Rabbit mAb

Clone No.SC05-85

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namesc Jun kinase 2 antibody
C Jun N terminal kinase 2 antibody
c-Jun N-terminal kinase 2 antibody
JNK 55 antibody
JNK-55 antibody
JNK2 alpha antibody
JNK2 antibody
JNK2 beta antibody
JNK2A antibody
JNK2alpha antibody
JNK2B antibody
JNK2BETA antibody
Jun kinase antibody
MAP kinase 9 antibody
MAPK 9 antibody
Mapk9 antibody
Mitogen activated protein kinase 9 antibody
Mitogen-activated protein kinase 9 antibody
MK09_HUMAN antibody
P54a antibody
p54aSAPK antibody
PRKM9 antibody
Protein kinase, mitogen-activated, 9 antibody
SAPK alpha antibody
SAPK antibody
SAPK1a antibody
Stress activated protein kinase 1a antibody
Stress-activated protein kinase JNK2 antibody

Accession NoSwiss-Prot#:P45984

Uniprot P45984

Gene ID 5601;

Calculated MW46/54 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of JNK2 on SHG-44 cell lysates using anti-JNK2 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-JNK2 antibody. Counter stained with hematoxylin.
ICC staining JNK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining JNK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining JNK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with JNK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1, and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2, and JNK3β2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38.

If you have published an article using product 48922, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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