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Location： Home > Recombinant Rabbit Monoclonal Antibodies > UBC9 Rabbit mAb

UBC9 Rabbit mAb#48933

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Product Detail

Product NameUBC9 Rabbit mAb

Clone No.SC0534

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, CHIP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesC358B7.1 antibody
p18 antibody
SUMO 1 protein ligase antibody
SUMO conjugating enzyme UBC9 antibody
SUMO-conjugating enzyme UBC9 antibody
SUMO-protein ligase antibody
SUMO1 protein ligase antibody
UBC9 antibody
UBC9_HUMAN antibody
UBCE9 antibody
Ube2i antibody
Ubiquitin carrier protein 9 antibody
Ubiquitin carrier protein antibody
Ubiquitin carrier protein I antibody
Ubiquitin conjugating enzyme 9 antibody
Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody
Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody
Ubiquitin conjugating enzyme UbcE2A antibody
Ubiquitin like protein SUMO 1 conjugating enzyme antibody
Ubiquitin protein ligase E2I antibody
Ubiquitin-conjugating enzyme E2 I antibody
Ubiquitin-protein ligase I antibody

Accession NoSwiss-Prot#:P63279

Uniprot P63279

Gene ID 7329;

Calculated MW18 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of UBC9 on different lysates using anti-UBC9 antibody at 1/1,000 dilution. Positive control:
Lane 1: HepG2
Lane 2: HUVEC
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. Counter stained with hematoxylin.
ICC staining UBC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining UBC9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation.

If you have published an article using product 48933, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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