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Wilms Tumor Protein Rabbit mAb#48959

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Product Detail

Product NameWilms Tumor Protein Rabbit mAb

Clone No.SC06-41

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms

Immunogen DescA synthesized peptide derived from human WT1

ConjugateUnconjugated

Other NamesWIT 2 antibody
WT 1 antibody
AWT1 antibody
FWT1 antibody
GUD antibody
NPHS4 antibody
WAGR antibody
Wilms tumor 1 antibody
Wilms Tumor antibody
Wilms tumor protein antibody
Wilms' tumor gene antibody
Wilms' tumor protein antibody
WIT2 antibody
WT antibody
WT1 antibody
WT1_HUMAN antibody
WT33 antibody

Accession NoSwiss-Prot#:P19544

Uniprot P19544

Gene ID 7490;

Calculated MW55 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details
WB 1:1000-1:2000
IHC 1:100-1:200
ICC/IF 1:50-1:200

FC 1:20-1:1000

ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
Western blot analysis of WT1 expression in K562 cell lysate.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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