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Location： Home > Recombinant Rabbit Monoclonal Antibodies > eIF5A Rabbit mAb

eIF5A Rabbit mAb#48963

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Product Detail

Product NameeIF5A Rabbit mAb

Clone No.SC60-09

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NameseIF 4D antibody
eIF 5A 1 antibody
EIF 5A antibody
eIF 5A1 antibody
eIF-4D antibody
eIF-5A antibody
eIF-5A-1 antibody
eIF-5A1 antibody
eIF4D antibody
eif5a antibody
EIF5A1 antibody
eIF5AI antibody
Eukaryotic initiation factor 5A antibody
Eukaryotic initiation factor 5A isoform 1 antibody
Eukaryotic translation initiation factor 5A 1 antibody
Eukaryotic translation initiation factor 5A antibody
Eukaryotic translation initiation factor 5A-1 antibody
IF5A1_HUMAN antibody
MGC104255 antibody
MGC99547 antibody
Rev binding factor antibody
Rev-binding factor antibody
uORF A antibody
uORF antibody

Accession NoSwiss-Prot#:P63241

Uniprot P63241

Gene ID 1984;

Calculated MW18 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of eIF5A on different lysates using anti-eIF5A antibody at 1/1,000 dilution. Positive control:
Lane 1: Jurkat
Lane 2: MCF-7
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
ICC staining eIF5A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining eIF5A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with eIF5A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The human eukaryotic translation initiation factor 5A gene, also designated eIF-4D or eIF5A, maps to chromosome 17p13.1 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A's biochemical activity and for cellular proliferative signaling. In addition, eIF5A is a cellular cofactor for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). Inhibition of eIF5A interaction with Rev leads to a block of the viral replication cycle.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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