Product Detail
Product NameSonic Hedgehog Protein/SHH(C-Product) Rabbit mAb
Clone No.SC05-28
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, IHC, FC
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesHHG 1 antibody HHG-1 antibody HHG1 antibody HLP 3 antibody HLP3 antibody Holoprosencephaly 3 antibody HPE 3 antibody HPE3 antibody MCOPCB5 antibody shh antibody SHH_HUMAN antibody SMMC I antibody SMMCI antibody Sonic Hedgehog (Drosophila) homolog antibody sonic hedgehog homolog (Drosophila) antibody Sonic hedgehog homolog antibody Sonic hedgehog protein antibody Sonic hedgehog protein C-product antibody TPT antibody TPTPS antibody
Accession NoSwiss-Prot#:Q15465
Uniprot
Q15465
Gene ID
6469;
Calculated MW50 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
IHC: 1:200-1:500
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.
ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Sonic Hedgehog Protein in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Sonic Hedgehog Protein in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Drosophila segment polarity gene hedgehog (hh) encodes a precursor protein which undergoes autocleavage to generate amino- and carboxy-terminal peptides. Both proteins are secreted and appear to function in embryonic and imaginal disc patterning. Several vertebrate homologs of Drosophila hh have been identified. These include Sonic hedgehog (Shh) (alternatively designated Vhh-1), Desert hedgehog (Dhh) and Indian hedgehog (Ihh). Each contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Shh resembles Drosophila hh in that it is processed to generate an amino-terminal secreted peptide that is retained at or near the cell surface and a carboxy-terminal glycosylated more diffusible peptide.
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