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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Daxx Rabbit mAb

Daxx Rabbit mAb#48986

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Product Detail

Product NameDaxx Rabbit mAb

Clone No.SC54-06

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesBING 2 antibody
BING2 antibody
CENP-C binding protein antibody
DAP 6 antibody
DAP6 antibody
DAXX antibody
DAXX_HUMAN antibody
Death associated protein 6 antibody
Death domain associated protein 6 antibody
Death domain associated protein antibody
Death domain-associated protein 6 antibody
EAP 1 antibody
EAP1 antibody
ETS1 associated protein 1 antibody
ETS1-associated protein 1 antibody
Fas binding protein antibody
Fas death domain associated protein antibody
Fas death domain-associated protein antibody
hDaxx antibody
MGC126245 antibody
MGC126246 antibody

Accession NoSwiss-Prot#:Q9UER7

Uniprot Q9UER7

Gene ID 1616;

Calculated MW81 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000

ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: A549
Lane 3: SW480
ICC staining Daxx in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Daxx in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Daxx antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Activation of the cell surface receptor FAS by FAS ligand leads to the initiation of apoptosis, a process necessary for the regulation of the immune system and tissue homeostasis. FAS-mediated apoptosis appears to involve a number of divergent and overlapping pathways. Daxx appears to be a central component of a FAS-mediated apoptotic pathway involving the activation of Jun N-terminal kinase (JNK). Although Daxx itself does not contain a death domain, it specifically binds to the death domain of FAS. Overexpression of Daxx activates the JNK pathway and enhances FAS-mediated apoptosis. The Daxx apoptotic pathway acts cooperatively with but is distinct from the FAS-mediated pathway that involves interactions between the death domain-containing protein FADD and the cysteine protease FLICE. Unlike the FAS-FADD-FLICE pathway, the Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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