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RALBP1 Rabbit mAb#49006

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Product Detail

Product NameRALBP1 Rabbit mAb

Clone No.SC06-49

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesRLIP1 antibody
76 kDa Ral-interacting protein antibody
76-kDa Ral-interacting protein antibody
Dinitrophenyl S-glutathione ATPase antibody
DNP-SG ATPase antibody
Ral-interacting protein 1 antibody
Ral-interacting protein 1, 76-KD antibody
RalA-binding protein 1 antibody
RalBP1 antibody
RBP1_HUMAN antibody
RIP1 antibody
RLIP1 antibody
RLIP76 antibody

Accession NoSwiss-Prot#:Q15311

Uniprot Q15311

Gene ID 10928;

Calculated MW95 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200


FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of 293 cells with RALBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells. A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain. This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42. A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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