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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Hes1 Rabbit mAb

Hes1 Rabbit mAb#49016

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Product NameHes1 Rabbit mAb

Clone No.SC06-21

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesbHLHb39 antibody
C-HAIRY1 antibody
c-hairy1A antibody
Class B basic helix-loop-helix protein 39 antibody
FLJ20408 antibody
Hairy and enhancer of split 1 (Drosophila) antibody
Hairy and enhancer of split 1 antibody
Hairy homolog (Drosophila) antibody
Hairy homolog antibody
Hairy like antibody
Hairy, Drosophila, homolog of antibody
Hairy-like protein antibody
Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
HAIRY1 antibody
HES-1 antibody
hes1 antibody
Hes1 hairy and enhancer of split 1 (Drosophila) antibody
HES1_HUMAN antibody
HHL antibody
HL antibody
HRY antibody
MGC129109 antibody
OTTHUMP00000209031 antibody
RHL antibody
Transcription factor HES-1 antibody

Accession NoSwiss-Prot#:Q14469

Uniprot Q14469

Gene ID 3280;

Calculated MW30 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100

Western blot analysis of Hes1 on different lysates using anti-Hes1 antibody at 1/1,000 dilution. Positive control:
Lane 1: MCF-7
Lane 2: SH-SY-5Y
ICC staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
ICC staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.

The Drosophila Hairy and enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homolog of Drosophila Hairy and enhancer of split are the HES gene family members HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homolog of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues.

If you have published an article using product 49016, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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