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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MUC1 Rabbit mAb

MUC1 Rabbit mAb#49024

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Product Detail

Product NameMUC1 Rabbit mAb

Clone No.SR4527

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesADMCKD antibody
ADMCKD1 antibody
Breast carcinoma associated antigen DF3 antibody
Breast carcinoma-associated antigen DF3 antibody
CA 15-3 antibody
CA15 3 antibody
CA15 3 antigen antibody
CA15.3 antibody
Cancer antigen 15-3 antibody
Carcinoma associated mucin antibody
Carcinoma-associated mucin antibody
CD 227 antibody
CD227 antibody
DF3 antigen antibody
EMA antibody
Episialin antibody
H23 antigen antibody
H23AG antibody
KL 6 antibody
KL-6 antibody
KL6 antibody
Krebs von den Lungen-6 antibody
MAM 6 antibody
MAM6 antibody
MCD antibody
MCKD antibody
MCKD1 antibody
Medullary cystic kidney disease 1 (autosomal dominant) antibody
Medullary cystic kidney disease, autosomal dominant antibody
MUC 1 antibody
MUC-1 antibody
MUC-1/SEC antibody
MUC-1/X antibody
MUC1 antibody
MUC1-alpha antibody
MUC1-beta antibody
MUC1-CT antibody
MUC1-NT antibody
MUC1/ZD antibody
MUC1_HUMAN antibody
Mucin 1 antibody
Mucin 1 transmembrane antibody
Mucin 1, cell surface associated antibody
Mucin-1 subunit beta antibody
Peanut reactive urinary mucin antibody
Peanut-reactive urinary mucin antibody
PEM antibody
PEMT antibody
Polymorphic epithelial mucin antibody
PUM antibody
Tumor associated epithelial membrane antigen antibody
Tumor associated epithelial mucin antibody
Tumor associated mucin antibody
Tumor-associated epithelial membrane antigen antibody
Tumor-associated mucin antibody

Accession NoSwiss-Prot#:P15941

Uniprot P15941

Gene ID 4582;

Calculated MW23 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.
ICC staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major com-ponent of various mucus gels and is broadly expressed in normal and tumor cells.

If you have published an article using product 49024, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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