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IKK alpha Rabbit mAb#49025

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Product NameIKK alpha Rabbit mAb

Clone No.SN06-99

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Nameschuk antibody
CHUK1 antibody
Conserved Helix Loop Helix Ubiquitous Kinase antibody
Conserved helix loop ubiquitous kinase antibody
Conserved helix-loop-helix ubiquitous kinase antibody
I Kappa B Kinase 1 antibody
I Kappa B Kinase Alpha antibody
I-kappa-B kinase 1 antibody
I-kappa-B kinase alpha antibody
IkappaB kinase antibody
IkB kinase alpha subunit antibody
IkBKA antibody
IKK 1 antibody
IKK A antibody
IKK a kinase antibody
IKK-A antibody
IKK-alpha antibody
IKK1 antibody
IKKA antibody
IKKA_HUMAN antibody
Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody
Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody
Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
NFKBIKA antibody
Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody
Nuclear factor NF kappa B inhibitor kinase alpha antibody
Nuclear factor NF-kappa-B inhibitor kinase alpha antibody
Nuclear factor NFkappaB inhibitor kinase alpha antibody
Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody
TCF-16 antibody
TCF16 antibody
Transcription factor 16 antibody

Accession NoSwiss-Prot#:O15111

Uniprot O15111

Gene ID 1147;

Calculated MW85 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of IKK alpha on Jurkat cells lysates using anti-IKK alpha antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-IKK alpha antibody. Counter stained with hematoxylin.
ICC staining IKK alpha in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with IKK alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase a (IKKa), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkB-a on Ser 32 and 36, the sites that trigger its degradation. IKKa appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKa is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKa, IKKb and IKKg (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

If you have published an article using product 49025, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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