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IKK alpha + IKK beta Rabbit mAb#49034

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Product NameIKK alpha + IKK beta Rabbit mAb

Clone No.SN63-02

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCHUK antibody
Conserved helix loop helix ubiquitous kinase antibody
I kappa B kinase 1 antibody
I kappa B kinase 2 antibody
I Kappa B kinase alpha antibody
I Kappa B kinase beta antibody
IkB kinase alpha subunit antibody
IkBKA antibody
IkBKB antibody
IKK a kinase antibody
IKK alpha antibody
IKK beta antibody
IKK1 antibody
IKK2 antibody
IKKA antibody
IKKB antibody
IMD15 antibody
Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody
Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
NFKBIKA antibody
NFKBIKB antibody
Nuclear factor NF kappa B inhibitor kinase beta antibody
Nuclear factor NFkappaB inhibitor kinase alpha antibody
TCF 16 antibody
TCF16 antibody
Transcription factor 16 antibody

Accession NoSwiss-Prot#:O14920

Uniprot O14920

Gene ID 3551;

Calculated MW85/87 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000

ICC: 1:100-1:500

Western blot analysis of IKK alpha+IKK beta on different lysates using anti-IKK alpha+IKK beta antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Daudi
Lane 3: A431
ICC staining IKK alpha+IKK beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining IKK alpha+IKK beta in B-6F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining IKK alpha+IKK beta in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase a (IKKa), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkB-a on Ser 32 and 36, the sites that trigger its degradation. IKKa appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKa is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKa, IKKb and IKKg (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

If you have published an article using product 49034, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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