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Location： Home > Recombinant Rabbit Monoclonal Antibodies > RAD18 Rabbit mAb

RAD18 Rabbit mAb#49044

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Product NameRAD18 Rabbit mAb

Clone No.SN66-01

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names2810024C04Rik antibody
DNA repair protein rad18 antibody
E3 ubiquitin-protein ligase RAD18 antibody
EC 6.3.2.- antibody
hHR 18 antibody
hHR18 antibody
hRAD 18 antibody
hRAD18 antibody
MGC156682 antibody
Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
Postreplication repair protein hRAD18p antibody
Postreplication repair protein RAD18 antibody
RAD 18 antibody
RAD18 antibody
RAD18 homolog (S. cerevisiae) antibody
RAD18 homolog antibody
RAD18 S. cerevisiae homolog antibody
RAD18 S. cerevisiae homolog of antibody
RAD18 transcript variant antibody
RAD18_HUMAN antibody
Rad18sc antibody
Radiation sensitivity protein 18 antibody
RING finger protein 73 antibody
RNF 73 antibody
RNF73 antibody
Structural maintenance of chromosomes protein 6 antibody
YCR066W antibody
YCR66W antibody

Accession NoSwiss-Prot#:Q9NS91

Uniprot Q9NS91

Gene ID 56852;

Calculated MW56/90kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution. Positive control:
Lane 1: NIH/3T3
Lane 2: 293T
Lane 3: MCF-7
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RAD18 antibody. Counter stained with hematoxylin.
ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining RAD18 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining RAD18 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with RAD18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
The RING-type zinc finger protein Rad18 is essential in post-replication repair of damaged DNA and contributes to the maintenance of genomic stability. Rad18 maintains chromosomal DNA with the Rad54-dependent DNA repair pathway and recruits ubiquitin-conjugating enzymes in the Rad6 pathway. Rad18 functions in gap-filling of a daughter strand on replication of damaged DNA and is localized in the nucleus.

If you have published an article using product 49044, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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