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Location： Home > Recombinant Rabbit Monoclonal Antibodies > AGR2 Rabbit mAb

AGR2 Rabbit mAb#49048

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Product Detail

Product NameAGR2 Rabbit mAb

Clone No.SN74-01

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, IP, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAG 2 antibody
AG 2 protein antibody
AG-2 antibody
AG2 antibody
AG2 protein antibody
AGR 2 antibody
AGR2 antibody
AGR2_HUMAN antibody
Anterior gradient 2 homolog (Xenopus laevis) antibody
anterior gradient 2 homolog antibody
Anterior gradient 2, Xenopus, homolog of antibody
Anterior gradient homolog 2 antibody
Anterior gradient protein 2 homolog antibody
GOB 4 antibody
GOB4 antibody
HAG 2 antibody
hAG-2 antibody
hAG2 antibody
HAG2/R antibody
HPC 8 antibody
hPC8 antibody
PDIA17 antibody
Protein disulfide isomerase family A member 17 antibody
secreted cement gland homolog antibody
Secreted cement gland protein XAG 2 homolog antibody
Secreted cement gland protein XAG-2 homolog antibody
Secreted cement gland protein XAG2 homolog antibody
XAG 2 antibody
XAG2 antibody

Accession NoSwiss-Prot#:O95994

Uniprot O95994

Gene ID 10551;

Calculated MW17 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-AGR2 antibody. Counter stained with hematoxylin.
ICC staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AGR2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AGR2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with AGR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
AGR2 (Anterior Gradient Protein 2), also known as AG2, GOB-4 or HAG-2, is a member of the anterior gradient homolog family. It is the human ortholog of XAG-2, the secreted Xenopus laevis Anterior Gradient protein. In X. laevis, it is involved in cement gland differentiation and neural marker gene expression. AGR2 is a secretory protein encoded by two different AGR2 transcripts. It interacts with LYPD3 and α-dystroglycan (DAG-1). AGR2 is ubiquitously expressed with up-regulated expression in prostate cancer, breast cancer, lung cancer, renal carcinomas and endometrial carcinomas. AGR2 expression is positively correlated with that of the estrogen receptor (ER) and is negatively correlated with that of the epidermal growth factor receptor (EGFR). AGR2 may serve as a potential therapeutic marker for various cancers.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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