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ATG5 Rabbit mAb#49050

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Product Detail

Product NameATG5 Rabbit mAb

Clone No.SN73-07

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAPG 5 antibody
APG 5L antibody
APG5 antibody
APG5 autophagy 5 like antibody
APG5 like antibody
APG5-like antibody
APG5L antibody
Apoptosis specific protein antibody
Apoptosis-specific protein antibody
ASP antibody
ATG 5 antibody
Atg5 antibody
ATG5 autophagy related 5 homolog antibody
ATG5_HUMAN antibody
Autophagy protein 5 antibody
Autophagy related 5 antibody
hAPG5 antibody
Homolog of S Cerevisiae autophagy 5 antibody
OTTHUMP00000040507 antibody

Accession NoSwiss-Prot#:Q9H1Y0

Uniprot Q9H1Y0

Gene ID 9474;

Calculated MW55 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

ICC staining ATG5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATG5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ATG5 antibody. Counter stained with hematoxylin.
Western blot analysis of ATG5 on different lysates using anti-ATG5 antibody at 1/1,000 dilution. Positive control:
Lane 1: Raji
Lane 2: Hela
Lane 3: PC12

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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