Product Detail
Product NameNSD3 Rabbit mAb
Clone No.SN07-11
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesDKFZp667H044 antibody FLJ20353 antibody Histone lysine N methyltransferase NSD3 antibody Histone-lysine N-methyltransferase NSD3 antibody MGC126766 antibody MGC142029 antibody NSD 3 antibody NSD3_HUMAN antibody Nuclear set domain containing 3 antibody Nuclear SET domain containing protein 3 antibody Nuclear SET domain-containing protein 3 antibody pp14328 antibody Protein whistle antibody Whistle antibody WHSC1 like protein 1 antibody WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody WHSC1-like protein 1 antibody WHSC1L1 antibody WHSC1L1 protein antibody Wolf Hirschhorn syndrome candidate 1 like 1 antibody Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody
Accession NoSwiss-Prot#:Q9BZ95
Uniprot
Q9BZ95
Gene ID
54904;
Calculated MW162 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
FC: 1:50-1:100
Western blot analysis of NSD3 on different lysates using anti-NSD3 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: 293T
Flow cytometric analysis of Hela cells with NSD3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
The deduced 1,437 amino acid NSD3 protein contains two PWWP domains involved in protein-protein interactions, five PHD-type zinc finger motifs found in chromatin-associated proteins, a SAC (SET-associated cys-rich) domain, a SET domain and a C-terminal C5HCH domain. Two NSD3 variants have been identified. The short variant comprised of 645 amino acids, arises from alternative polyadenylation and exon splicing and contains a single PWWP domain. A longer NSD3 variant, which is only expressed in HeLa cells, is comprised of 1,388 amino acid residues. The human WHSC1L1 gene, which encodes the NSD3 protein, shares 68% and 55% identity with mouse Nsd1 and human WHSC1, respectively. Highest expression of NSD3 is observed in brain, heart and skeletal muscle tissues; lower levels of NSD3 expression are observed in the liver and lungs.
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