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SOX9 Rabbit mAb#49070

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Product Detail

Product NameSOX9 Rabbit mAb

Clone No.SN74-09

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namescampomelic dysplasia autosomal sex reversal antibody
CMD 1 antibody
CMD1 antibody
CMPD 1 antibody
CMPD1 antibody
SOX 9 antibody
Sox9 antibody
SOX9_HUMAN antibody
SRA 1 antibody
SRA1 antibody
SRXX2 antibody
SRXY10 antibody
SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
SRY (sex determining region Y) box 9 antibody
SRY (sex determining region Y)-box 9 antibody
SRY (sex-determining region Y)-box 9 protein antibody
SRY related HMG box gene 9 antibody
Transcription factor SOX 9 antibody
Transcription factor SOX-9 antibody
transcription factor SOX9 antibody

Accession NoSwiss-Prot#:P48436

Uniprot P48436

Gene ID 6662;

Calculated MW56 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SOX9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SOX9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SOX9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-SOX9 antibody. Counter stained with hematoxylin.
ICC staining SOX9 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SOX9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with SOX9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

If you have published an article using product 49070, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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