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Location： Home > Recombinant Rabbit Monoclonal Antibodies > ABCF1 Rabbit mAb

ABCF1 Rabbit mAb#49078

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Product Detail

Product NameABCF1 Rabbit mAb

Clone No.SN07-12

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Rt

Immunogen DescSynthetic peptide within aa 796-845 of Human ABCF1

ConjugateUnconjugated

Other NamesABC27 antibody
ABC50 antibody
ABCF1 antibody
ABCF1_HUMAN antibody
ATP binding cassette 50 (TNF alpha stimulated) antibody
ATP binding cassette 50 antibody
ATP binding cassette sub family F member 1 antibody
ATP binding cassette, sub family F (GCN20), member 1 antibody
ATP-binding cassette 50 antibody
ATP-binding cassette sub-family F member 1 antibody
AU041969 antibody
D17Wsu166e antibody
EST123147 antibody
GCN20 antibody
TNF alpha stimulated ABC protein antibody
TNF-alpha-stimulated ABC protein antibody
TNFalpha inducible ATP binding protein antibody
wu:fb79c06 antibody
wu:fc39a05 antibody
wu:fj94a08 antibody
zgc:85667 antibody

Accession NoSwiss-Prot#:Q8NE71

Uniprot Q8NE71

Gene ID 23;

Calculated MW96 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

Western blot analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
ICC staining ABCF1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ABCF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ABCF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ABCF1 (ATP-binding cassette sub-family F member 1, TNF-alpha-stimulated ABC protein) is a 845 amino acid protein encoded by the human gene ABCF1. ABCF1 belongs to the ABC transporter family (EF3 subfamily) and contains two ABC transporter domains. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. ABCF1 is believed to have a role in mRNA translation due to its interaction with eukaryotic initiation factor 2 (eIF2). It is also associated with ribosomes. ABCF1 is ubiquitously expressed and can be induced with TNF. Upon DNA damage, ABCF1 is phosphorylated by either ATM or ATR.

If you have published an article using product 49078, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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