Product Detail
Product NameSIRT2 Rabbit mAb
Clone No.SN70-04
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsICC/IF, IP, FC
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesFLJ35621 antibody
FLJ37491 antibody
NAD dependent deacetylase sirtuin 2 antibody
NAD-dependent deacetylase sirtuin-2 antibody
NAD-dependent protein deacetylase sirtuin-2 antibody
Regulatory protein SIR2 homolog 2 antibody
Silencing information regulator 2 like antibody
Silent information regulator 2 antibody
SIR2 antibody
SIR2 like protein 2 antibody
Sir2 related protein type 2 antibody
SIR2, S. cerevisiae, homolog-loke 2 antibody
SIR2-like protein 2 antibody
SIR2L antibody
SIR2L2 antibody
SIRT2 antibody
SIRT2_HUMAN antibody
Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody
Sirtuin 2 antibody
Sirtuin type 2 antibody
Accession NoSwiss-Prot#:Q8IXJ6
Uniprot
Q8IXJ6
Gene ID
22933;
Calculated MW43 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
ICC: 1:50-1:200
FC: 1:50-1:100
ICC staining SIRT2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SIRT2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with SIRT2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
The silent information regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in a NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT proteins may function via mono-ADP-ribosylation of proteins. SIRT2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis.
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