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CD10 Rabbit mAb#49098

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Product Detail

Product NameCD10 Rabbit mAb

Clone No.SR4530

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, FC, IHC, ICC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAtriopeptidase antibody
CALLA antibody
CD10 antibody
CD10 antigen antibody
Common acute lymphocytic leukemia antigen antibody
DKFZp686O16152 antibody
EC 3.4.24.11 antibody
Enkephalinase antibody
EPN antibody
Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
Membrane metallo endopeptidase antibody
Membrane metallo endopeptidase variant 1 antibody
Membrane metallo endopeptidase variant 2 antibody
Membrane metalloendopeptidase antibody
Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
Membrane metalloendopeptidase variant 1 antibody
Membrane metalloendopeptidase variant 2 antibody
MGC126681 antibody
MGC126707 antibody
MME antibody
NEP antibody
NEP_HUMAN antibody
Neprilysin antibody
neprilysin-390 antibody
neprilysin-411 antibody
Neutral endopeptidase 24.11 antibody
Neutral endopeptidase antibody
Neutral endopeptidase, membrane-associated antibody
SFE antibody
Skin fibroblast elastase antibody

Accession NoSwiss-Prot#:P08473

Uniprot P08473

Gene ID 4311;

Calculated MW100 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1000
IHC:1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
ICC staining CD10 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with CD10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
CD10, also called the common acute lymphoblastic leukemia antigen (CALLA) and neutral endopeptidase (NEP), is a type II integral membrane glycoprotein. CD10 acts as a zinc metalloprotease that cleaves a variety of biologically active peptides including Angiotensins I and II. It is expressed on early B and T lymphoid precursors, B blasts, some granulocytes, bone marrow stromal cells and certain epithelial cells including some tumor cell lines. CD10 is used as a marker of common acute lymphocytic leukemias and some lymphomas.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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