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TAPA1/CD81 Rabbit mAb#49103

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Product Detail

Product NameTAPA1/CD81 Rabbit mAb

Clone No.SR4531

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names26 kDa cell surface protein TAPA 1 antibody
26 kDa cell surface protein TAPA-1 antibody
26 kDa cell surface protein TAPA1 antibody
CD 81 antibody
CD81 antibody
CD81 antigen (target of antiproliferative antibody
1) antibody
CD81 antigen antibody
CD81 molecule antibody
CD81_HUMAN antibody
CVID6 antibody
S5.7 antibody
TAPA 1 antibody
TAPA1 antibody
Target of the antiproliferative antibody
1 antibody
Tetraspanin 28 antibody
Tetraspanin-28 antibody
Tetraspanin28 antibody
Tspan 28 antibody
Tspan-28 antibody
Tspan28 antibody

Accession NoSwiss-Prot#:P60033

Uniprot P60033

Gene ID 975;

Calculated MW20 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100

Western blot analysis of CD81 on different lysates using anti-CD81 antibody at 1/1,000 dilution. Positive control:
Lane 1: JAR
Lane 2: PC-12
Lane 3: K562
Flow cytometric analysis of Jurkat cells with CD81 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
ICC staining CD81 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD81 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD81 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD81 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD81 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD81 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD81 antibody. Counter stained with hematoxylin.

CD81, also called TAPA-1, is a type III transmembrane protein that is broadly expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin. CD81 is believed to be involved in both cell growth and signal transduction. It can be present as a multimolecular complex in association with CD37 and/or CD53, or on the surface of B cells in association with CD19, CD21 and/or MHC class II antigens.

If you have published an article using product 49103, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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