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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Ret Rabbit mAb

Ret Rabbit mAb#49115

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Product Detail

Product NameRet Rabbit mAb

Clone No.SN20-28

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesC ret antibody
Cadherin family member 12 antibody
Cadherin related family member 16 antibody
CDHF 12 antibody
CDHF12 antibody
CDHR16 antibody
ELKS Fusion gene antibody
HSCR 1 antibody
HSCR1 antibody
Hydroxyaryl protein kinase antibody
MEN2A antibody
MEN2B antibody
MTC 1 antibody
MTC1 antibody
Multiple endocrine neoplasia and medullary thyroid carcinoma 1 antibody
Oncogene RET antibody
Proto oncogene tyrosine protein kinase receptor ret antibody
Proto-oncogene c-Ret antibody
Proto-oncogene tyrosine-protein kinase receptor ret antibody
PTC antibody
RET antibody
RET ELE1 antibody
Ret Proto oncogene antibody
RET transforming sequence antibody
RET_HUMAN antibody
RET51 antibody
RET9 antibody
tyrosine-protein kinase receptor ret antibody

Accession NoSwiss-Prot#:P07949

Uniprot P07949

Gene ID 5979;

Calculated MW150/175 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of Ret on MCF-7 cells lysates using anti-Ret antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Ret antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Ret antibody. Counter stained with hematoxylin.
ICC staining Ret in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Ret in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Ret in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SW480 cells with Ret antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicomponent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors. Ligands that bind the Ret receptor include the glial cell line-derived neurotrophic factor (GDNF) and its congeners neurturin, persephin, and artemin. Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprungs disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity.

If you have published an article using product 49115, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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