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MEK3 Rabbit mAb#49128

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Product NameMEK3 Rabbit mAb

Clone No.SD20-93

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAW212142 antibody
dual specificity mitogen activated protein kinase kinase 3 antibody
Dual specificity mitogen-activated protein kinase kinase 3 antibody
MAP kinase kinase 3 antibody
map2k3 antibody
MAPK ERK kinase 3 antibody
MAPK/ERK kinase 3 antibody
MAPKK 3 antibody
MAPKK3 antibody
MEK 3 antibody
MEK3 antibody
Mitogen activated protein kinase kinase 3 antibody
MKK 3 antibody
MKK3 antibody
mMKK3b antibody
MP2K3_HUMAN antibody
PRKMK 3 antibody
PRKMK3 antibody
protein kinase, mitogen-activated, kinase 3 antibody
SAPK kinase 2 antibody
SAPKK 2 antibody
SAPKK2 antibody
Stress activated protein kinase kinase 2 antibody

Accession NoSwiss-Prot#:P46734

Uniprot P46734

Gene ID 5606;

Calculated MW39 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of MEK3 on Hela lysates using anti-MEK3 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MEK3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-MEK3 antibody. Counter stained with hematoxylin.
ICC staining MEK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with MEK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

If you have published an article using product 49128, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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