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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Cyclin B2 Rabbit mAb

Cyclin B2 Rabbit mAb#49130

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Product Detail

Product NameCyclin B2 Rabbit mAb

Clone No.SD2045

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namesccnb2 antibody
CCNB2_HUMAN antibody
CycB2 antibody
Cyclin B2 antibody
G2 mitotic specific cyclin B2 antibody
G2/mitotic specific cyclin B2 antibody
G2/mitotic-specific cyclin-B2 antibody
HsT17299 antibody
MGC108931 antibody
MGC140694 antibody

Accession NoSwiss-Prot#:O95067

Uniprot O95067

Gene ID 9133;

Calculated MW45 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Cyclin B2 on different lysates using anti-Cyclin B2 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: K562
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin B2 antibody. Counter stained with hematoxylin.
ICC staining Cyclin B2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cyclin B2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex . Evidence has established that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B-type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

If you have published an article using product 49130, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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