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Location： Home > Recombinant Rabbit Monoclonal Antibodies > STAT1 alpha Rabbit mAb

STAT1 alpha Rabbit mAb#49131

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Product Detail

Product NameSTAT1 alpha Rabbit mAb

Clone No.SD20-75

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCANDF7 antibody
DKFZp686B04100 antibody
ISGF 3 antibody
ISGF3 antibody
OTTHUMP00000163552 antibody
OTTHUMP00000165046 antibody
OTTHUMP00000165047 antibody
OTTHUMP00000205845 antibody
Signal transducer and activator of transcription 1 antibody
Signal transducer and activator of transcription 1, 91kDa antibody
Signal transducer and activator of transcription 1-alpha/beta antibody
Stat1 antibody
STAT1_HUMAN antibody
STAT91 antibody
Transcription factor ISGF-3 components p91/p84 antibody

Accession NoSwiss-Prot#:P42224

Uniprot P42224

Gene ID 6772;

Calculated MW87 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of STAT1 on different lysates using anti-STAT1 alpha antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: 293
Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-STAT1 alpha antibody. Counter stained with hematoxylin.
ICC staining STAT1 alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining STAT1 alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining STAT1 alpha in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with STAT1 alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

If you have published an article using product 49131, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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