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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Histone H2B Rabbit mAb

Histone H2B Rabbit mAb#49134

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Product Detail

Product NameHistone H2B Rabbit mAb

Clone No.SD20-63

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesH2B GL105 antibody
H2B histone family member O antibody
H2B histone family member S antibody
H2B.1 antibody
H2B.1 B antibody
H2B.b antibody
H2B.c antibody
H2B.d antibody
H2B.e antibody
H2B.f antibody
H2B.j antibody
H2B.q antibody
H2B/b antibody
H2B/c antibody
H2B/d antibody
H2B/e antibody
H2B/f antibody
H2B/j antibody
H2B/o antibody
H2B/q antibody
H2BFB antibody
H2BFC antibody
H2BFD antibody
H2BFE antibody
H2BFF antibody
H2BFJ antibody
H2BFO antibody
H2BFQ antibody
H2BFS antibody
HIRIP2 antibody
HIST1H2BB antibody
HIST1H2BD antibody
HIST1H2BH antibody
HIST1H2BL antibody
HIST1H2BM antibody
HIST1H2BN antibody
HIST2H2BE antibody
Histone H2B antibody
Histone H2B type 1 B antibody
Histone H2B type 1 D antibody
Histone H2B type 1 H antibody
Histone H2B type 1 L antibody
Histone H2B type 1 M antibody
Histone H2B type 1 N antibody
Histone H2B type 2 E antibody
histone protein antibody

Accession NoSwiss-Prot#:O60814

Uniprot O60814

Gene ID 85236;

Calculated MW14 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of Histone H2B on different lysates using anti-Histone H2B antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: PC12
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.
ICC staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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