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Location： Home > Recombinant Rabbit Monoclonal Antibodies > AP2M1 Rabbit mAb

AP2M1 Rabbit mAb#49143

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Product Detail

Product NameAP2M1 Rabbit mAb

Clone No.SD0746

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAdapter-related protein complex 2 mu subunit antibody
Adaptin mu 1 antibody
Adaptin-mu2 antibody
Adaptor protein complex AP 2 subunit mu antibody
Adaptor protein complex AP-2 subunit mu antibody
Adaptor related protein complex 2 mu 1 subunit antibody
AP 2 mu 2 chain antibody
AP-2 complex subunit mu antibody
AP-2 mu chain antibody
Ap2m1 antibody
AP2M1_HUMAN antibody
AP50 antibody
CLAPM1 antibody
Clathrin adaptor complex AP2 mu subunit antibody
Clathrin assembly protein complex 2 medium chain antibody
Clathrin associated/assembly/adaptor protein medium 1 antibody
Clathrin coat adaptor protein AP50 antibody
Clathrin coat assembly protein AP50 antibody
Clathrin coat associated protein AP50 antibody
Clathrin coat-associated protein AP50 antibody
HA2 50 kDa subunit antibody
mu2 antibody
Plasma membrane adaptor AP-2 50 kDa protein antibody

Accession NoSwiss-Prot#:Q96CW1

Uniprot Q96CW1

Gene ID 1173;

Calculated MW50 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AP2M1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AP2M1 antibody. Counter stained with hematoxylin.
ICC staining AP2M1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AP2M1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining AP2M1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of PANC-1 cells with AP2M1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Adaptins are heterotetrameric subunits of adaptors, which are complexes involved in the formation of Clathrin-coated pits for vesicle-mediated endocytosis. Clathrin and its associated heterotetrameric protein complexes make up the main protein components of the coat surrounding the cytoplasmic face of coated vesicles. The Adaptin family, comprising a, b, b' and g classes, is also responsible for the transport of ligand-receptor complexes from plasma membranes and the trans-Golgi network to lysosomes. Two main types of adaptor proteins (APs), AP-1 and AP-2, are found in Clathrin-coated structures located at the Golgi complex and the plasma membrane of mammalian cells, respectively. Adaptor protein complex 2 (AP-2) is composed of two large Adaptins (a1A/AP2A1 and b1/AP2B1), a medium Adaptin (m2/AP-2m1) and a small Adaptin (s2 long/AP2S1). AP-2m1, a 435 amino acid protein, links Clathrin to receptors in coated vesicles.

If you have published an article using product 49143, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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