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MEK7 Rabbit mAb#49145

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Product Detail

Product NameMEK7 Rabbit mAb

Clone No.SD20-87

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namesc-Jun N-terminal kinase kinase 2 antibody
Dual specificity mitogen activated protein kinase kinase 7 antibody
Dual specificity mitogen-activated protein kinase kinase 7 antibody
JNK activating kinase 2 antibody
JNK kinase 2 antibody
JNK-activating kinase 2 antibody
JNKK 2 antibody
Jnkk-2 antibody
Jnkk2 antibody
MAP kinase kinase 7 antibody
MAP2K7 antibody
MAPK/ERK kinase 7 antibody
MAPKK 7 antibody
MAPKK-7 antibody
MAPKK7 antibody
MEK 7 antibody
Mitogen Activated Protein Kinase kinase 7 antibody
MKK 7 antibody
MKK-7 antibody
MKK7 antibody
MP2K7_HUMAN antibody
PRKMK 7 antibody
PRKMK-7 antibody
PRKMK7 antibody
SAPK kinase 4 antibody
SAPKK-4 antibody
SAPKK4 antibody
Sek 2 antibody
Sek-2 antibody
Sek2 antibody
SKK4 antibody
stress-activated protein kinase kinase 4 antibody

Accession NoSwiss-Prot#:O14733

Uniprot O14733

Gene ID 5609;

Calculated MW47 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: A431
Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MEK7 antibody. Counter stained with hematoxylin.
ICC staining MEK7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MEK7 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MEK7 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with MEK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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