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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Histone H4 Rabbit mAb

Histone H4 Rabbit mAb#49154

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Product Detail

Product NameHistone H4 Rabbit mAb

Clone No.SD209-04

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesdJ160A22.1 antibody
dJ160A22.2 antibody
dJ221C16.1 antibody
dJ221C16.9 antibody
FO108 antibody
H4 antibody
H4.k antibody
H4/a antibody
H4/b antibody
H4/c antibody
H4/d antibody
H4/e antibody
H4/g antibody
H4/h antibody
H4/I antibody
H4/j antibody
H4/k antibody
H4/m antibody
H4/n antibody
H4/p antibody
H4_HUMAN antibody
H4F2 antibody
H4F2iii antibody
H4F2iv antibody
H4FA antibody
H4FB antibody
H4FC antibody
H4FD antibody
H4FE antibody
H4FG antibody
H4FH antibody
H4FI antibody
H4FJ antibody
H4FK antibody
H4FM antibody
H4FN antibody
H4M antibody
HIST1H4A antibody
HIST1H4B antibody
HIST1H4C antibody
HIST1H4D antibody
HIST1H4E antibody
HIST1H4F antibody
HIST1H4H antibody
HIST1H4I antibody
HIST1H4J antibody
HIST1H4K antibody
HIST1H4L antibody
HIST2H4 antibody
HIST2H4A antibody
Hist4h4 antibody
Histone 1 H4a antibody
Histone 1 H4b antibody
Histone 1 H4c antibody
Histone 1 H4d antibody
Histone 1 H4e antibody
Histone 1 H4f antibody
Histone 1 H4h antibody
Histone 1 H4i antibody
Histone 1 H4j antibody
Histone 1 H4k antibody
Histone 1 H4l antibody
Histone 2 H4a antibody
histone 4 H4 antibody
Histone H4 antibody
MGC24116 antibody

Accession NoSwiss-Prot#:P62805

Uniprot P62805

Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370;

Calculated MW20 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Histone H4 on mouse spleen lysates using anti-Histone H4 antibody at 1/5,000 dilution.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.
ICC staining Histone H4 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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