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KAP1 Rabbit mAb#49167

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Product Detail

Product NameKAP1 Rabbit mAb

Clone No.SD081-05

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesE3 SUMO protein ligase TRIM28 antibody
E3 SUMO-protein ligase TRIM28 antibody
FLJ29029 antibody
KAP 1 antibody
KAP-1 antibody
KRAB associated protein 1 antibody
KRAB interacting protein 1 antibody
KRAB-associated protein 1 antibody
KRAB-interacting protein 1 antibody
KRIP 1 antibody
KRIP-1 antibody
KRIP1 antibody
Nuclear corepressor KAP 1 antibody
Nuclear corepressor KAP-1 antibody
RING finger protein 96 antibody
RNF96 antibody
TF1B antibody
TIF1 beta antibody
TIF1-beta antibody
TIF1B antibody
TIF1B_HUMAN antibody
Transcription intermediary factor 1 beta antibody
Transcription intermediary factor 1-beta antibody
Trim28 antibody
Tripartite motif containing 28 antibody
tripartite motif containing protein 28 antibody
Tripartite motif-containing protein 28 antibody

Accession NoSwiss-Prot#:Q13263

Uniprot Q13263

Gene ID 10155;

Calculated MW110 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:2,000-1:5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of KAP1 on different lysates using anti-KAP1 antibody at 1/1,000 dilution. Positive control:
Lane 1: HepG2
Lane 2: Hela
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KAP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-KAP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-KAP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-KAP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KAP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-KAP1 antibody. Counter stained with hematoxylin.
ICC staining KAP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with KAP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
TIF1β, for transcriptional intermediary factor 1-beta, also designated KAP1(for KRAB-associated protein 1), TF1β and TRIM28 (for tripartif motif-containing 28), is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain. Like TIF1α, TIF1β contains both a Cys/His PHD (plant homeodomain) finger and bromodomain that form a cooperative unit required for transcriptional repression. TIF1β mediates transcriptional control by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors and by binding DNA through its zinc finger. The human TIF1β gene maps to human chromosome 19q13.4 and encodes an 835 amino acid nuclear protein.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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