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Cdc34 Rabbit mAb#49217

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Product Detail

Product NameCdc34 Rabbit mAb

Clone No.JJ086-07

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCdc 34 antibody
Cdc34 antibody
Cell division cycle 34 antibody
Cell division cycle 34 homolog (S. cerevisiae) antibody
Cell division cycle 34 homolog antibody
E2 CDC34 antibody
UB2R1_HUMAN antibody
UBC 3 antibody
UBC3 antibody
UBCH3 antibody
UBE2 R1 antibody
UBE2R1 antibody
Ubiquitin carrier protein antibody
Ubiquitin conjugating enzyme Cdc34 antibody
Ubiquitin conjugating enzyme E2 32 kDa complementing antibody
Ubiquitin protein ligase antibody
Ubiquitin protein ligase R1 antibody
Ubiquitin-conjugating enzyme E2 R1 antibody
Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody
Ubiquitin-conjugating enzyme E2-CDC34 antibody
Ubiquitin-protein ligase R1 antibody

Accession NoSwiss-Prot#:P49427

Uniprot P49427

Gene ID 997;

Calculated MW34 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000

ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution. Positive control:
Lane 1: K562
Lane 2: Jurkat
ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdc34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdc34 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with Cdc34 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

If you have published an article using product 49217, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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