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HDAC8 Rabbit mAb#49219

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Product Detail

Product NameHDAC8 Rabbit mAb

Clone No.JJ0845

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCDA07 antibody
CDLS5 antibody
HD 8 antibody
HD8 antibody
HDAC 8 antibody
Hdac8 antibody
HDAC8_HUMAN antibody
HDACL 1 antibody
HDACL1 antibody
Histone deacetylase 8 antibody
Histone deacetylase like 1 antibody
MRXS6 antibody
RPD 3 antibody
RPD3 antibody
WTS antibody

Accession NoSwiss-Prot#:Q9BY41

Uniprot Q9BY41

Gene ID 55869;

Calculated MW42 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: K562
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
ICC staining HDAC8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining HDAC8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining HDAC8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with HDAC8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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