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Location： Home > Recombinant Rabbit Monoclonal Antibodies > pro Caspase 9 Rabbit mAb

pro Caspase 9 Rabbit mAb#49230

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Product Detail

Product Namepro Caspase 9 Rabbit mAb

Clone No.JJ08-05

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAPAF-3 antibody
APAF3 antibody
Apoptotic protease Mch-6 antibody
Apoptotic protease-activating factor 3 antibody
CASP-9 antibody
CASP9 antibody
CASP9_HUMAN antibody
Caspase-9 subunit p10 antibody
ICE-LAP6 antibody
ICE-like apoptotic protease 6 antibody
ICELAP6 antibody
MCH6 antibody

Accession NoSwiss-Prot#:P55211

Uniprot P55211

Gene ID 842;

Calculated MW46 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200


FC: 1:10-1:50
Western blot analysis of pro Caspase 9 on Hela cells lysates using anti-pro Caspase 9 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-pro Caspase 9 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Jurkat cells with pro Caspase 9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, Ced-3/caspase-1, is comprised of caspase-1, caspase-2, caspase-3, caspase-4, caspase-6, caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9 and caspase-10. Ced-3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the nuclear protein PARP into an apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins, which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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