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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Cdk7 Rabbit mAb

Cdk7 Rabbit mAb#49233

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Product Detail

Product NameCdk7 Rabbit mAb

Clone No.JJ203-01

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names39 kDa protein kinase antibody
CAK antibody
CAK1 antibody
Cdk activating kinase antibody
CDK-activating kinase 1 antibody
CDK-activating kinase antibody
cdk7 antibody
CDK7_HUMAN antibody
CDKN7 antibody
Cell division protein kinase 7 antibody
Cyclin dependent kinase 7 antibody
cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
Cyclin-dependent kinase 7 antibody
HCAK antibody
Homolog of Xenopus MO15 Cdk activating kinase antibody
Kinase subunit of CAK antibody
MO15 antibody
MO15, Xenopus, homolog of antibody
P39 Mo15 antibody
p39MO15 antibody
Serine threonine kinase Stk1 antibody
Serine/threonine protein kinase 1 antibody
Serine/threonine protein kinase MO15 antibody
STK1 antibody
TFIIH basal transcription factor complex kinase subunit antibody

Accession NoSwiss-Prot#:P50613

Uniprot P50613

Gene ID 1022;

Calculated MW39 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.
ICC staining Cdk7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk7 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A431 cells with Cdk7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires additional phosphorylation at Thr 160. The enzyme responsible for the phosphorylation of Cdk2 on Thr 160 and also of Cdc2 p34 on Thr 161, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine residue required for full activity; mutation of this residue severely reduces CAK activity.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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