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Location： Home > Recombinant Rabbit Monoclonal Antibodies > MEK3+MEK6 Rabbit mAb

MEK3+MEK6 Rabbit mAb#49258

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Product Detail

Product NameMEK3+MEK6 Rabbit mAb

Clone No.JJ087-09

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesDual specificity mitogen activated protein kinase kinase 6 antibody
Dual specificity mitogen-activated protein kinase kinase 3 antibody
MAP kinase kinase 3 antibody
MAP kinase kinase 6 antibody
map2k3 antibody
MAP2K6 antibody
MAPK/ERK kinase 3 antibody
MAPK/ERK kinase 6 antibody
MAPKK 3 antibody
MAPKK 6 antibody
MAPKK3 antibody
MAPKK6 antibody
MEK 3 antibody
MEK 6 antibody
Mitogen activated protein kinase kinase 3 antibody
Mitogen activated protein kinase kinase 6 antibody
MKK3 antibody
MKK6 antibody
MP2K3_HUMAN antibody
PRKMK3 antibody
PRKMK6 antibody
Protein kinase, mitogen activated, kinase 6 (MAP kinase kinase 6) antibody
SAPK kinase 2 antibody
SAPKK 3 antibody
SAPKK-2 antibody
SAPKK2 antibody
SAPKK3 antibody
Stress activated protein kinase kinase 3 antibody
Stress-activated protein kinase kinase 2 antibody

Accession NoSwiss-Prot#:P46734

Uniprot P46734

Gene ID 5606;

Calculated MW39/37 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Jurkat
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK3+MEK6 antibody. Counter stained with hematoxylin.
ICC staining MEK3+MEK6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MEK3+MEK6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MEK3+MEK6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with MEK3+MEK6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

If you have published an article using product 49258, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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