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PAX5 Rabbit mAb#49259

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Product Detail

Product NamePAX5 Rabbit mAb

Clone No.JJ08-87

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesB cell lineage specific activator antibody
B cell lineage specific activator protein antibody
B cell specific activator protein antibody
B cell specific transcription factor antibody
B-cell-specific transcription factor antibody
BSAP antibody
EBB-1 antibody
KLP antibody
Paired box 5 antibody
Paired box gene 5 (B cell lineage specific activator protein) antibody
Paired box gene 5 (B cell lineage specific activator) antibody
Paired box gene 5 antibody
Paired box homeotic gene 5 antibody
Paired box protein Pax 5 antibody
Paired box protein Pax-5 antibody
Paired domain gene 5 antibody
PAX 5 antibody
PAX5 antibody
PAX5_HUMAN antibody
Transcription factor PAX 5 antibody

Accession NoSwiss-Prot#:Q02548

Uniprot Q02548

Gene ID 5079;

Calculated MW42 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of PAX5 on Daudi cells lysates using anti-PAX5 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PAX5 antibody. Counter stained with hematoxylin.
ICC staining PAX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PAX5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PAX5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Raji cells with PAX5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Pax family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax family genes are also involved in a variety of signal transduction pathways in the adult organism. Mutations in Pax proteins have been linked to disease and cancer in humans. For example, the human PAX5 gene encodes a B cell lineage-specific protein, Pax-5, also designated B cell specific activator protein or BSAP, which is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. Pax-5 functions to regulate not only B cell development, but also influences the balance between immunoglobulin secretion and B cell proliferation. Overexpression of Pax-5 has been implicated in cellular transformation, and in the case of small lymphocytic lymphomas with plasmacytoid differentiation, a t(9;14)(p13;q32) translocation resulting in the deregulation of PAX5 gene expression has been detected.

If you have published an article using product 49259, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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