Product Detail
Product NamePAX8 Rabbit mAb
Clone No.JJ08-88
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, IHC
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesOTTHUMP00000158659 antibody
OTTHUMP00000158660 antibody
OTTHUMP00000203723 antibody
OTTHUMP00000203724 antibody
Paired box 8 antibody
Paired box gene 8 antibody
paired box homeotic gene 8 antibody
Paired box protein Pax 8 antibody
Paired box protein Pax-8 antibody
Paired domain gene 8 antibody
PAX 8 antibody
PAX8 antibody
PAX8_HUMAN antibody
Accession NoSwiss-Prot#:Q06710
Uniprot
Q06710
Gene ID
7849;
Calculated MW48 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of PAX8 on SKOV-3 cells lysates using anti-PAX8 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-PAX8 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PAX8 antibody. Counter stained with hematoxylin.
ICC staining PAX8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The Pax family encodes transcription factors that function during embryogenesis and regulate the temporal and position-dependent differentiation of cells. Pax-8 is expressed in the developing and adult thyroid, the developing secretory system and at lower levels, in the adult kidney. Pax-8 complexes with TTF-1 and TTF-2 to induce thyroid follicular cell differentiation and thyroid hormone biosynthesis by regulating the expression of sodium iodide symporter (NIS), thyroid peroxidase (TPO), thyroglobulin (TG) and the thyrotropin receptor (TSHR). Treatment of FRTL-5 cells with TGFβ1 decreases Pax-8 mRNA levels and Pax-8 DNA binding activity, which suppresses the expression of TG and the formation of thyrocytes. Patients who have autosomal dominant mutations of the Pax-8 gene develop thyroid dysgenesis. The Pax-8 gene produces six isoforms, A to F, that are generated by alternative splicing and differ in their carboxy-terminal regions. The Pax-8 isoforms display different DNA binding capacities and are thought to be functionally distinct. The gene which encodes Pax-8 maps to human chromosome 2q12-q14.
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