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CD3D Rabbit mAb#49268

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Product Detail

Product NameCD3D Rabbit mAb

Clone No.SR4533

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB;IHC;ICC/IF;IP;FC

Species ReactivityHu

Immunogen DescA synthesized peptide derived from human CD3 delta

ConjugateUnconjugated

Other NamesCD3 antigen delta subunit antibody
CD3 delta antibody
CD3d antibody
CD3d antigen delta polypeptide (TiT3 complex) antibody
CD3d molecule delta (CD3-TCR complex) antibody
CD3D_HUMAN antibody
IMD19 antibody
OKT3 delta chain antibody
T cell receptor T3 delta chain antibody
T-cell receptor T3 delta chain antibody
T-cell surface glycoprotein CD3 delta chain antibody
T3D antibody

Accession NoSwiss-Prot#:P04234

Uniprot P04234

Gene ID 915;

Calculated MW19 kDa

Concentration0.5mg/ml

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details
WB 1:1000-1:2000
IHC 1:100-1:200
ICC/IF 1:50-1:200
IP 1:20-1:50
FC 1:20-1:100
Flow cytometric analysis of Raji cells with CD3D antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human spleen, using CD3D Antibody.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis of Jurkat cells, using CD3D Antibody.

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.

If you have published an article using product 49268, please notify us so that we can cite your literature.

et al,ScRNA-seq reveals novel immune-suppressive T cells and investigates CMV-TCR-T cells cytotoxicity against GBM. In J Immunother Cancer on 2024 Apr 30 by Xinmiao Long, Zuping Zhang, et al..PMID:38688579 , (2024),
PMID: 38688579
et al,ScRNA-seq reveals novel immunesuppressive T cells and investigates CMV-TCR-T cells cytotoxicity against GBM , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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