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Location： Home > Recombinant Rabbit Monoclonal Antibodies > CDC42 Rabbit mAb

CDC42 Rabbit mAb#49282

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Product Detail

Product NameCDC42 Rabbit mAb

Clone No.JJ086-04

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB IHC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCDC42 antibody
CDC42_HUMAN antibody
CDC42Hs antibody
Cell division control protein 42 homolog antibody
Cell division cycle 42 (GTP binding protein 25kDa) antibody
Cell division cycle 42 antibody
dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
G25K antibody
G25K GTP-binding protein antibody
Growth regulating protein antibody
GTP binding protein 25kDa antibody
Small GTP binding protein CDC42 antibody
TKS antibody

Accession NoSwiss-Prot#:P60953

Uniprot P60953

Gene ID 998;

Calculated MWPredicted band size: 21 kDa

Sdspage MWObserved band size: 21 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes: CDC42 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : C6 whole cell lysates
Lane 4 : Mouse brain lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 3 seconds
All lanes:CDC42 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : CDC42 knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon tissue stained for CDC42 using 49282 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse skin tissue stained for CDC42 using 49282 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat skin tissue stained for CDC42 using 49282 at 1/100 dilution in immunohistochemical analysis.

The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division. One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42.

If you have published an article using product 49282, please notify us so that we can cite your literature.

et al,A transcribed ultraconserved noncoding RNA, uc.285+, promotes colorectal cancer proliferation through dual targeting of CDC42 by directly binding mRNA and protein. In Transl Res on 2024 Aug by Sixian Chen, Qingyun Zhao,et al..PMID:38552953 , (2024),
PMID: 38552953
et al,A transcribed ultraconserved noncoding RNA, uc.285+, promotes colorectal cancer proliferation through dual targeting of CDC42 by directly binding mRNA and protein , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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