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Location： Home > Recombinant Rabbit Monoclonal Antibodies > E2F1 Rabbit mAb

E2F1 Rabbit mAb#49286

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Product Detail

Product NameE2F1 Rabbit mAb

Clone No.JJ092-02

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen DescA synthesized peptide derived from human E2F1

ConjugateUnconjugated

Other NamesDmel\CG6376 antibody
Dmel_CG6376 antibody
drosE2F1 antibody
E(Sev-CycE)3A antibody
E(var)3-93E antibody
E2-promoter binding facto antibody
E2F 1 antibody
E2F transcription factor 1 antibody
E2F-1 antibody
E2f-PA antibody
E2f-PB antibody
E2f-PC antibody
E2F1 antibody
E2f1 E2F transcription factor 1 antibody
E2F1_HUMAN antibody
Evar(3)164 antibody
KIAA4009 antibody
l(3)07172 antibody
l(3)j3B1 antibody
l(3)j3C2 antibody
l(3)rM729 antibody
mKIAA4009 antibody
OTTHUMP00000030661 antibody
PBR3 antibody
PRB binding protein E2F 1 antibody
PRB-binding protein E2F-1 antibody
RBAP 1 antibody
RBAP-1 antibody
RBAP1 antibody
RBBP-3 antibody
RBBP3 antibody
RBP 3 antibody
RBP3 antibody
Retinoblastoma-associated protein 1 antibody
Retinoblastoma-binding protein 3 antibody
Transcription factor E2F1 antibody

Accession NoSwiss-Prot#:Q01094

Uniprot Q01094

Gene ID 1869;

Calculated MW65 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB 1:1000-1:2000;
IHC 1:100-1:200;
ICC/IF 1:50-1:200;
IP 1:20-1:50;
FC 1:20-1:100
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.
ICC staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.

If you have published an article using product 49286, please notify us so that we can cite your literature.

et al,The ATPase Pontin is a key cell cycle regulator by amplifying E2F1 transcription response in glioma , (2021),
PMID: 33542204

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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