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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Actin Rabbit mAb

Actin Rabbit mAb#49294

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Product Detail

Product NameActin Rabbit mAb

Clone No.JJ09-29

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu, Ms, Rt, zebrafish

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namesa actin antibody
ACTA antibody
ACTA1 antibody
Actin alpha skeletal muscle antibody
Actin antibody
actin, alpha 1, skeletal muscle 1 antibody
actin, alpha 1, skeletal muscle antibody
Actin, alpha skeletal muscle antibody
actina antibody
actine antibody
ACTS_HUMAN antibody
aktin antibody
Alpha Actin 1 antibody
Alpha skeletal muscle Actin antibody
alpha skeletal muscle antibody
alpha-actin antibody
Alpha-actin-1 antibody
ASMA antibody
CFTD antibody
CFTD1 antibody
CFTDM antibody
MPFD antibody
NEM1 antibody
NEM2 antibody
NEM3 antibody
nemaline myopathy type 3 antibody

Accession NoSwiss-Prot#:P68133

Uniprot P68133

Gene ID 58;

Calculated MW42 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control:
Lane 1: zebrafish
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
:Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control:  Lane 1:Hybrid fish (crucian-carp) brain tissue         Lane 2:Hybrid fish (crucian-carp) kidney tissue 

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.
ICC staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

If you have published an article using product 49294, please notify us so that we can cite your literature.

et al,GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation. In Cell Discov on 2022 Aug 9 by Shupei Wei, Qin Zhao, et al..PMID:35945223 , (2022),
PMID: 35945223
et al,Increasing cisplatin exposure promotes small-cell lung cancer transformation after a shift from glucose metabolism to fatty acid metabolism. , (2025),
PMID: 40155472

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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