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Location： Home > Recombinant Rabbit Monoclonal Antibodies > PDIA6 Rabbit mAb

PDIA6 Rabbit mAb#49340

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Product Detail

Product NamePDIA6 Rabbit mAb

Clone No.JF0974

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesEndoplasmic reticulum protein 5 antibody
ER protein 5 antibody
ERp5 antibody
P5 antibody
Pdia6 antibody
PDIA6_HUMAN antibody
Protein disulfide isomerase A6 antibody
Protein disulfide isomerase associated 6 antibody
Protein disulfide isomerase family A member 6 antibody
Protein disulfide isomerase P5 antibody
Protein disulfide isomerase related protein antibody
Protein disulfide-isomerase A6 antibody
Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
Thioredoxin domain containing protein 7 antibody
Thioredoxin domain-containing protein 7 antibody
TXNDC7 antibody

Accession NoSwiss-Prot#:Q15084

Uniprot Q15084

Gene ID 10130;

Calculated MW48 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000

ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of PDIA6 on different lysates using anti-PDIA6 antibody at 1/1,000 dilution. Positive control:
Lane 1: HepG2
Lane 2: K562
ICC staining PDIA6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PDIA6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PDIA6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with PDIA6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Endoplasmic reticulum proteins (ERps) are widely expressed proteins that localize to the ER and may act as proteases, protein disulfide isomerases, thiol-disulfide oxidases or phospholipases. ERp5, also known as PDIA6 (protein disulfide isomerase family A, member 6) or TXNDC7 is a 440 amino acid protein that contains two thioredoxin domains and belongs to the protein disulfide isomerase family. Localized to the melanosome, as well as to the lumen of the endoplasmic reticulum, ERp5 functions to catalyze the rearrangement of disulfide bonds in a variety of different proteins. Via its catalytic activity, ERp5 is able to reduce the disulfide bond that binds MICA to tumor cells, thereby releasing MICA and reducing the rate of tumor expansion. Multiple isoforms of ERp5 exist due to alternative splicing events.

If you have published an article using product 49340, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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