Product Detail
Product NameGAPDH(HRP conjugated) Rabbit mAb
Clone No.JF81-04
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB
Species ReactivityHu, Ms, Rt, Monkey, Chicken, Fish
Immunogen Descrecombinant protein
ConjugateHRP
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Accession NoSwiss-Prot#:P04406
Uniprot
P04406
Gene ID
2597;
Calculated MW36 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000
Western blot analysis of GAPDH on different lysates using anti-GAPDH antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: NIH/3T3
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the β-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins.
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