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Location： Home > Recombinant Rabbit Monoclonal Antibodies > beta Actin(HRP conjugated) Rabbit mAb

beta Actin(HRP conjugated) Rabbit mAb#49381

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Product Detail

Product Namebeta Actin(HRP conjugated) Rabbit mAb

Clone No.JF53-10

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB

Species ReactivityHu, Ms, Rt, Monkey, Hamster, Plant, zebrafish

Immunogen Descrecombinant protein

ConjugateHRP

Other NamesA26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
Beta-actin antibody
BRWS1 antibody
E430023M04Rik antibody
MGC128179 antibody
PS1TP5 binding protein 1 antibody
PS1TP5BP1 antibody

Accession NoSwiss-Prot#:P60709

Uniprot P60709

Gene ID 60;

Calculated MW42 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000



Western blot analysis of beta Actin on different lysates using anti-beta Actin antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: NIH/3T3
Lane 4: zebrafish
Western blot analysis of beta Actin on hybrid fish (crucian-carp) brain tissue lysate using anti-beta Actin antibody at 1/500 dilution.

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

If you have published an article using product 49381, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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