Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Recombinant Rabbit Monoclonal Antibodies > ASPP2 Rabbit mAb

ASPP2 Rabbit mAb#49393

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameASPP2 Rabbit mAb

Clone No.JF09-98

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names53BP2 antibody
Apoptosis stimulating of p53 protein 2 antibody
Apoptosis stimulating protein of p53 2 antibody
Apoptosis-stimulating of p53 protein 2 antibody
ASPP2 antibody
ASPP2_HUMAN antibody
BBP antibody
Bcl2 binding protein antibody
Bcl2-binding protein antibody
NY REN 51 antigen antibody
p53 binding protein 2 antibody
p53-binding protein 2 antibody
p53BP2 antibody
PPP1R13A antibody
Renal carcinoma antigen NY-REN-51 antibody
Tp53bp2 antibody
Tumor protein p53 binding protein 2 antibody
Tumor suppressor p53 binding protein 2 antibody
Tumor suppressor p53-binding protein 2 antibody

Accession NoSwiss-Prot#:Q13625

Uniprot Q13625

Gene ID 7159;

Calculated MW150 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of ASPP2 on different lysates using anti-ASPP2 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: MCF-7
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ASPP2 antibody. Counter stained with hematoxylin.
ICC staining ASPP2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ASPP2 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.

If you have published an article using product 49393, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors