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Location： Home > Recombinant Rabbit Monoclonal Antibodies > CD19 Rabbit mAb

CD19 Rabbit mAb#49407

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Product Detail

Product NameCD19 Rabbit mAb

Clone No.JF100-06

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Namesantibody
deficiency due to defect in CD19, included antibody
AW495831 antibody
B lymphocyte antigen CD19 antibody
B lymphocyte surface antigen B4 antibody
B-lymphocyte antigen CD19 antibody
B-lymphocyte surface antigen B4 antibody
B4 antibody
CD19 antibody
CD19 antigen antibody
CD19 molecule antibody
Cd19 protein antibody
CD19_HUMAN antibody
CVID3 antibody
Differentiation antigen CD19 antibody
Leu 12 antibody
Leu-12 antibody
Leu12 antibody
MGC109570 antibody
MGC12802 antibody
T-cell surface antigen Leu-12 antibody

Accession NoSwiss-Prot#:P15391

Uniprot P15391

Gene ID 930;

Calculated MW95 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of CD19 on Raji cells lysates using anti-CD19 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD19 antibody. Counter stained with hematoxylin.
ICC staining CD19 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD19 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK). Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin b1, CD21 and CD81, which are involved in regulating B-cell development.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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