Product Detail
Product NameNG2 Rabbit mAb
Clone No.JM10-13
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, IHC, FC
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other Names4732461B14Rik antibody
AN2 antibody
AN2 proteoglycan antibody
Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody
Chondroitin sulfate proteoglycan 4 antibody
Chondroitin sulfate proteoglycan NG2 antibody
Cspg4 antibody
Cspg4 chondroitin sulfate proteoglycan 4 antibody
CSPG4_HUMAN antibody
HMW-MAA antibody
HSN tumor-specific antigen antibody
Kiaa4232 antibody
MCSP antibody
MCSPG antibody
MEL-CSPG antibody
Melanoma chondroitin sulfate proteoglycan antibody
Melanoma-associated chondroitin sulfate proteoglycan antibody
MELCSPG antibody
MSK16 antibody
NG2 antibody
Accession NoSwiss-Prot#:Q6UVK1
Uniprot
Q6UVK1
Gene ID
1464;
Calculated MW251 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NG2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NG2 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
NG2 (also known as melanoma-associated chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MSK16 and MEL-CSPG) stabilizes cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. NG2 may facilitate primary melanoma progression by enhancing the activation of key signaling pathways important for tumor invasion and growth. Threonine 2256 phosphorylation of rat NG2 (Threonine 2252 phosphorylation of human NG2) leads to redistribution of NG2 on the surface of astrocytomas, polarization of the cell and a significant increase in cell motility. NG2 acts as a co-receptor for spreading and focal contact formation in association with α4 β1 integrin in malignant melanoma cells. NG2 is present on blood vessels throughout the rat embryo. Microvessels within the rat CNS express NG2 on endothelial cells, and outside the CNS, NG2 is present on smooth muscle cells. NG2 is a novel marker for epidermal stem cells that contributes to their patterned distribution by promoting stem cell clustering.
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