Product Detail
Product NameCD59 Rabbit mAb
Clone No.JM10-71
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, FC,IP
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other Names16.3A5 antibody
1F5 antibody
1F5 antigen antibody
20 kDa homologous restriction factor antibody
CD 59 antibody
CD_antigen=CD59 antibody
CD59 antibody
CD59 antigen antibody
CD59 antigen complement regulatory protein antibody
CD59 antigen p18 20 antibody
CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
CD59 glycoprotein antibody
CD59 molecule antibody
CD59 molecule complement regulatory protein antibody
CD59_HUMAN antibody
Cd59a antibody
Complement regulatory protein antibody
EJ16 antibody
EJ30 antibody
EL32 antibody
FLJ38134 antibody
FLJ92039 antibody
G344 antibody
HRF 20 antibody
HRF-20 antibody
HRF20 antibody
Human leukocyte antigen MIC11 antibody
Ly 6 like protein antibody
Lymphocytic antigen CD59/MEM43 antibody
MAC inhibitory protein antibody
MAC IP antibody
MAC-inhibitory protein antibody
MAC-IP antibody
MACIF antibody
MACIP antibody
MEM43 antibody
MEM43 antigen antibody
Membrane attack complex (MAC) inhibition factor antibody
Membrane attack complex inhibition factor antibody
Membrane inhibitor of reactive lysis antibody
MGC2354 antibody
MIC11 antibody
MIN1 antibody
MIN2 antibody
MIN3 antibody
MIRL antibody
MSK21 antibody
p18 20 antibody
Protectin antibody
Surface antigen recognized by monoclonal antibody
16.3A5 antibody
T cell activating protein antibody
Accession NoSwiss-Prot#:P13987
Uniprot
P13987
Gene ID
966;
Calculated MW14/20 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
ICC: 1:100-1:500
IP: 1:10-1:50
FC: 1:50-1:100
Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution. Positive control: Lane 1: Human placenta Lane 2: HUVEC Lane 3: K562
ICC staining CD59 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD59 in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD59 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of NIH-3T3 cells with CD59 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
CD59 is a GPI-anchored glycoprotein that is expressed on leukocytes, vascular endothelial cells, various epithelial cells and placenta. CD59 acts together with CD58 in mediating T cell adhesion and activation, and it may be a second ligand of CD2. CD59 functions as a regulator of the terminal pathway of complement by binding to the C8/C9 components of the assembling membrane attack complex (MAC) on host cell membranes, to stop the formation of the lytic pore. CD59 also drives both calcium release and activation of lipid-raft associated signalling molecules such as tyrosine kinases. CD59 gene has two p53-responsive domains that may be implicated in the defense of host cells from damage by the complement system in inflammation, suggesting that p53 could be used to mediate susceptibility of tumor cells to the complement lysis during chemotherapy.
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