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ATPB Rabbit mAb#49442

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Product Detail

Product NameATPB Rabbit mAb

Clone No.JM10-90

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt, Zebrafish

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesATP 5B antibody
ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
ATP synthase subunit beta mitochondrial antibody
ATP synthase subunit beta, mitochondrial antibody
atp5b antibody
ATPB antibody
ATPB_HUMAN antibody
ATPMB antibody
ATPSB antibody
Epididymis secretory protein Li 271 antibody
HEL-S-271 antibody
Mitochondrial ATP synthase beta subunit antibody
Mitochondrial ATP Synthase Subunit Beta antibody
Mitochondrial ATP synthetase beta subunit antibody

Accession NoSwiss-Prot#:P06576

Uniprot P06576

Gene ID 506;

Calculated MW57 kDa

Sdspage MW53 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line2: HepG2 Line3: 293T
Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin.
ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATPB in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes : ATPB Rabbit mAb at 1/1k dilution
Lane 1 : Rat spleen lysates whole cell lysates
Lane 2 : Mouse spleen lysates whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 53 kDa

Exposure time: 6 seconds
All lanes : ATPB Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : ATPB knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

If you have published an article using product 49442, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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